Electrospray Ionization Mass Spectrometry
Enhanced charging, or supercharging, of analytes in electrospray ionization mass spectrometry (ESI MS) facilitates high resolution MS by reducing an ion mass-to-charge (m/z) ratio, increasing tandem mass spectrometry (MS/MS) efficiency.
Immobilization of Azide-Tagged Biomolecules
A photolabile o-nitrobenzyl linker-cyclooctyne conjugate was prepared, immobilized on poly (methacrylate) beads and utilized as a trap for azide-functionalized compounds. The reagent-free and time economic catch and release protocol was deemed useful for chemical proteomics applications.
Ligand Binding in the Non-Canonical Site
Pleckstrin homology (PH) domains are common membrane-targeting modules. Slm1-PH utilizes a non-canonical binding site to bind PtdInsPs. Additionally, Slm1-PH may have retained an active canonical site. The presence of both canonical and non-canonical binding pocket may account for the cooperative binding to PtdInsPs and DHS-1P.
From Protein Interaction Networks to Novel Therapeutic Strategies
Biological networks capture a variety of molecular interactions and provide an excellent opportunity to consider physiological characteristics of individual molecules within their cellular context. The pathogenic mechanisms trigger the onset and progression of diseases.
GeneCodis3
GeneCodis has become a valuable tool to functionally interpret results from experimental techniques in genomics. This web-based application integrates different sources of information to finding groups of genes with similar biological meaning. A new comparative analysis has been added to allow the differential analysis of gene sets.
